Transcriptome analysis of the diseased intervertebral disc tissue in patients with spinal tuberculosis

作者：Tian’en Xu, Wenjuan Fan, Cong Chen, Kai Feng, Xiaoyun Sheng, Hong Wang, Kai Yang, Bao Chen, Xu Wang, Yapeng Wang · 发表于：BMC Medical Genomics · 年份：2024 · DOI：10.1186/s12920-024-01981-w · 被引用次数：5 · 研究领域：Infectious Diseases and Tuberculosis、Spine and Intervertebral Disc Pathology、Spondyloarthritis Studies and Treatments

OBJECTIVE: To investigate the differential expression genes (DEGs) in spinal tuberculosis using transcriptomics, with the aim of identifying novel therapeutic targets and prognostic indicators for the clinical management of spinal tuberculosis. METHODS: Patients who visited the Department of Orthopedics at the Second Hospital, Lanzhou University from January 2021 to May 2023 were enrolled. Based on the inclusion and exclusion criteria, there were 5 patients in the test group and 5 patients in the control group. Total RNA was extracted and paired-end sequencing was conducted on the sequencing platform. After processing the sequencing data with clean reads and annotating the reference genome, FPKM normalization and differential expression analysis were performed. The DEGs and long non-coding RNAs (LncRNAs) were analyzed for Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment. The cis-regulation of differentially expressed mRNAs (DE mRNAs) by LncRNAs was predicted and analyzed to establish a co-expression network. RESULTS: This study identified 2366 DEGs, with 974 genes significantly upregulated and 1392 genes significantly downregulated. The upregulated genes are associated with cytokine-cytokine receptor interactions, tuberculosis, and TNF-α signaling pathways, primarily enriched in biological processes such as immunity and inflammation. The downregulated genes are related to muscle development, contraction, fungal defense response, and collagen me...