Effects of semaglutide on gut microbiota, cognitive function and inflammation in obese mice

作者：Jing Feng, Zhenjie Teng, Yang Yu, Jingzhen Liu, Shuchun Chen · 发表于：PeerJ · 年份：2024 · DOI：10.7717/peerj.17891 · 被引用次数：53 · 研究领域：Gut microbiota and health、Diabetes Treatment and Management、Diet and metabolism studies

Objective This study aims to investigate the effects of semaglutide on gut microbiota, cognitive function, and inflammation in obese mice. Method Twenty-four C57BL/6J male mice were randomly assigned to three groups: a normal-chow diet group (NCD, n = 8), high-fat diet group (HFD, n = 8), and HFD+semaglutide group (Sema, n = 8). The mice were fed a HFD to establish an animal model of obesity and then administered with semaglutide or saline for 12 weeks. Cognitive function was assessed using the Morris water maze test. Serum pro-inflammatory cytokines were measured. 16S rRNA gene sequencing technology was used to explore gut microbiota characteristics in obese mice. Result Obese mice showed significant cognitive impairment and inflammation. Semaglutide improved cognitive function and attenuated inflammation induced by a HFD diet. The abundance of gut microbiota was significantly changed in the HFD group, including decreased Akkermansia , Muribaculaceae , Coriobacteriaceae _UCG_002, Clostridia _UCG_014 and increased Romboutsia , Dubosiella , Enterorhabdus . Whereas semaglutide could dramatically reverse the relative abundance of these gut microbiota. Correlation analysis suggested that cognitive function was positively correlated with Muribaculaceae and Clostridia _UCG_014, and negatively associated with Romboutsia and Dubosiella . Romboutsia was positively correlated with TNFα, IL-6 and IL-1β. While Clostridia _UCG_014 was negatively related to TNFα, IL-6 and IL-1β. Conclusion...