Genetically Encoded Incorporation of IFN-α into Collagen-like Protein–Hyaluronic Acid Hydrogels for Diabetic Chronic Wound Healing

作者：Jie Wang, Jiaqi Li, Yinan Sun, Xing Liu, Liping Wang, Yan Xia, Jinxia Huang, Jian Q. Feng, Shuang Jia, Yimiao Li, Zhao Guo, Yuxin Dong, Liyao Wang, Xinyu Li · 发表于：ACS Materials Letters · 年份：2024 · DOI：10.1021/acsmaterialslett.4c01170 · 被引用次数：11 · 研究领域：Wound Healing and Treatments、Diabetic Foot Ulcer Assessment and Management、Tendon Structure and Treatment

The role of interferon-alpha is critical in modulating immune responses and the wound healing process. However, the rapid degradation and short lifespan of IFN-α in the body limit its therapeutic efficacy in wound management. This study presents an innovative approach to enhancing diabetic wound repair through the incorporation of IFN-α into double network hydrogels composed of hyaluronic acid and collagen-like protein (CLP). Two incorporation methods were evaluated: genetic fusion and spy-chemistry ligation. Our results demonstrate that IFN-α incorporated via spy-chemistry significantly outperformed genetic fusion in terms of cell viability, migration, and protein expression. In vivo studies further confirmed that spy-chemistry ligated IFN-α HA-CLP hydrogels markedly improved wound healing, as evidenced by elevated levels of COL-1α, CK-14, and α-SMA, compared to blank HA-CLP hydrogels and saline-treated controls. These findings underscore the potential of spy-chemistry ligated IFN-α-HA-CLP hydrogels as a promising therapeutic strategy for promoting effective wound healing in diabetic patients.