Genus-wide analysis of Trichoderma antagonism toward Pythium and Globisporangium plant pathogens and the contribution of cellulases to the antagonism

作者：Siqiao Chen, Paul Daly, Wilfred Mabeche Anjago, Rong Wang, Yishen Zhao, Xian Wen, Dongmei Zhou, Sheng Deng, Xisha Lin, Josef Voglmeir, Feng Cai, Qirong Shen, Irina S. Druzhinina, Lihui Wei · 发表于：Applied and Environmental Microbiology · 年份：2024 · DOI：10.1128/aem.00681-24 · 被引用次数：13 · 研究领域：Plant Pathogens and Resistance、Plant-Microbe Interactions and Immunity、Mycorrhizal Fungi and Plant Interactions

ABSTRACT Parasitism is an important lifestyle in the Trichoderma genus but has not been studied in a genus-wide way toward Pythium and Globisporangium hosts. Our approach screened a genus-wide set of 30 Trichoderma species in dual culture assays with two soil-borne Pythium and three Globisporangium plant-parasitic species and used exo-proteomic analyses, with the aim to correlate Trichoderma antagonism with potential strategies for attacking Pythium and Globisporangium . The Trichoderma spp. showed a wide range of antagonism from strong to weak, but the same Trichoderma strain showed similar levels toward all the Pythium and Globisporangium species. The Trichoderma enzymes from strong ( Trichoderma asperellum , Trichoderma atroviride , and Trichoderma virens ), moderate ( Trichoderma cf. guizhouense and Trichoderma reesei ), and weak ( Trichoderma parepimyces ) antagonists were induced by the autoclaved mycelia of one of the screened Pythium species, Pythium myriotylum . The variable proportions of putative cellulases, proteases, and redox enzymes suggested diverse as well as shared strategies amongst the antagonists. There was a partial positive correlation between antagonism from microscopy and the cellulase activity induced by autoclaved P. myriotylum mycelia in different Trichoderma species. The deletion of the cellulase transcriptional activator XYR1 in T. reesei led to lower antagonism toward Pythium and Globisporangium . The antagonism of Pythium and Globisporangium ap...