Functional Characterization of Peripheral Neurons and a Receptor Recognizing Sex Pheromones in Hyphantria cunea (Erebidae)

作者：Guiqiu Di, Zicong Li, Weichao Ma, Dun Jiang, Guirong Wang, Shanchun Yan · 发表于：Journal of Agricultural and Food Chemistry · 年份：2024 · DOI：10.1021/acs.jafc.4c02438 · 被引用次数：5 · 研究领域：Neurobiology and Insect Physiology Research、Insect and Arachnid Ecology and Behavior、Insect Pheromone Research and Control

Hyphantria cunea (Lepidoptera: Erebidae) is difficult and costly to control as a quarantine pest found globally. Sex pheromone trapping is an effective measure for its population monitoring and control; however, the peripheral neural mechanism of sex pheromone recognition in H. cunea remains unclear. An electrophysiological analysis showed that both male and female moths of H. cunea responded to four components of sex pheromones and the responses of male moths were stronger than those of the female moths. We identified three types of trichoid sensilla (ST) responsive to sex pheromones using the single sensillum recording technique. Each type was involved in recognizing 9 R, 10 S -epoxy-1, Z 3, Z 6-heneicosatriene (1, Z 3, Z 6–9 S, 10 R -epoxy-21Hy). Four peripheral neurons involved in the olfactory encoding of sex pheromones were identified. Four candidate pheromone receptor (PR) genes, HcunPR1a, HcunPR1b, HcunPR3, and HcunPR4, were screened by transcriptome sequencing. All of them were highly expressed in the antennae of males, except for HcunPR4, which was highly expressed in the antennae of females. Functional identification showed that HcunPR1a responded to sex pheromone. Other HcunPRs were not functionally identified. In summary, neurons involved in sex pheromone recognition of H. cunea were located in the ST, and HcunPR1a recognized secondary pheromone components 1, Z 3, Z 6–9 S, 10 R- epoxy-21Hy. Interestingly, PRs that recognize the main components of the sex pheromon...