Multi-functional properties of lactic acid bacteria strains derived from canine feces

作者：Yunjiang Liu, Jiali Wang, Haohong Zheng, Jialiang Xin, Zhijun Zhong, Haifeng Liu, Hualin Fu, Ziyao Zhou, Xianmeng Qiu, Guangneng Peng · 发表于：Frontiers in Veterinary Science · 年份：2024 · DOI：10.3389/fvets.2024.1404580 · 被引用次数：16 · 研究领域：Probiotics and Fermented Foods、Gut microbiota and health、Protein Hydrolysis and Bioactive Peptides

Introduction Probiotics, especially Lactic Acid Bacteria (LAB), can promote the health of host animals in a variety of ways, such as regulating intestinal flora and stimulating the host’s immune system. Methods In this study, 206 LAB strains were isolated from 48 canine fecal samples. Eleven LAB strains were selected based on growth performance, acid and bile salt resistance. The 11 candidates underwent comprehensive evaluation for probiotic properties, including antipathogenic activity, adhesion, safety, antioxidant capacity, and metabolites. Results The results of the antipathogenic activity tests showed that 11 LAB strains exhibited strong inhibitory effect and co-aggregation ability against four target pathogens ( E. coli , Staphylococcus aureus , Salmonella braenderup , and Pseudomonas aeruginosa ). The results of the adhesion test showed that the 11 LAB strains had high cell surface hydrophobicity, self-aggregation ability, biofilm-forming ability and adhesion ability to the Caco-2 cells. Among them, Lactobacillus acidophilus (L177) showed strong activity in various adhesion experiments. Safety tests showed that 11 LAB strains are sensitive to most antibiotics, with L102, L171, and L177 having the highest sensitivity rate at 85.71%, and no hemolysis occurred in all strains. Antioxidant test results showed that all strains showed good H 2 O 2 tolerance, high scavenging capacity for 1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH) and hydroxyl (OH − ). In addition, 11 LAB s...