Microbial synthesis of gallic acid and its glucoside β‐glucogallin

作者：Jiao Guo, Xiang Ren, Liangyu Lu, Ning An, Shuwei Li, Mingjie Geng, Geng Li, Xiaolin Shen, Xinxiao Sun, Jia Wang, Qipeng Yuan · 发表于：Biotechnology and Bioengineering · 年份：2024 · DOI：10.1002/bit.28820 · 被引用次数：20 · 研究领域：Tannin, Tannase and Anticancer Activities、Microbial Metabolism and Applications、Biofuel production and bioconversion

Gallic acid (GA) and β-glucogallin (BGG) are natural products with diverse uses in pharmaceutical, food, chemical and cosmetic industries. They are valued for their wide-ranging properties such as antioxidant, antibacterial, antidiabetic, and anticancer properties. Despite their significant importance, microbial production of GA and BGG faces challenges such as limited titers and yields, along with the incomplete understanding of BGG biosynthesis pathways in microorganisms. To address these challenges, we developed a recombinant Escherichia coli strain capable of efficiently producing GA. Our approach involved screening efficient pathway enzymes, integrating biosynthetic pathway genes into the genome while balancing carbon flux via adjusting expression levels, and strengthening the shikimate pathway to remove bottlenecks. The resultant strain achieved impressive results, producing 51.57 g/L of GA with a carbon yield of 0.45 g/g glucose and a productivity of 1.07 g/L/h. Furthermore, we extended this microbial platform to biosynthesize BGG by screening GA 1-O-glucosyltransferase, leading to the de novo production of 92.42 mg/L of BGG. This work establishes an efficient chassis for producing GA at an industrial level and provides a microbial platform for generating GA derivatives.