Phytofabrication, characterization of silver nanoparticles using Hippophae rhamnoides berries extract and their biological activities

作者：Neha Rana, A. Najitha Banu, Bimlesh Kumar, Sandeep Kumar Singh, Noha E. Abdel-razik, Naif A. Jalal, Farkad Bantun, Emanuel Vamanu, Mahendra Pratap Singh · 发表于：Frontiers in Microbiology · 年份：2024 · DOI：10.3389/fmicb.2024.1399937 · 被引用次数：25 · 研究领域：Phytochemical and Pharmacological Studies、Nanoparticles: synthesis and applications、Medicinal Plants and Bioactive Compounds

Introduction Fabrication of plant-based metal nanoparticles has yielded promising results, establishing this approach as viable, sustainable, and non-toxic in the biomedical sector for targeted drug delivery, diagnostic imaging, biosensing, cancer therapy, and antimicrobial treatments. Methods The present work demonstrates the suitability of Hippophae rhamnoides berries for the instant green synthesis of silver nanoparticles to check their antioxidant, lipid peroxidation, and antimicrobial potential. The preliminary characterization of Hippophae rhamnoides -mediated AgNPs was validated by monitoring the color shift in the solution from pale yellow to reddish brown, which was further confirmed by UV–vis spectroscopy and the plasmon peaks were observed at 450 nm. Field Emission Scanning Electron Microscopy (FESEM) and X-ray diffraction (XRD) were used to evaluate the surface topography and structure of AgNPs. Herein, the antioxidant potential of synthesized AgNPs was investigated using DPPH free radical assay and the antimicrobial efficacy of similar was checked against E. coli and S. aureus by following MIC (minimum inhibitory concentration) and MBC (Minimum bactericidal concentration) assay. Along with the inhibitory percentage of lipid peroxidation was analysed by following TBARS (Thiobarbituric acid reactive species) assay. Results & discussion The results revealed that the AgNPs were spherical in shape with an average size distribution within the range of 23.5–28 n...