Profiling of viral load, antibody and inflammatory response of people with monkeypox during hospitalization: a prospective longitudinal cohort study in China

作者：Li Guo, Rui Song, Qiao Zhang, Danyang Li, Lan Chen, Meiyu Fang, Yan Xiao, Xinming Wang, Yanan Li, Ru Gao, Zimeng Liu, Xiaoyou Chen, Zhixia Gu, Hongxin Zhao, Jingchuan Zhong, Xueqi Chi, Guanying Wang, Yuanyuan Zhang, Ning Han, Ronghua Jin, Xiang Ren, Jianwei Wang · 发表于：EBioMedicine · 年份：2024 · DOI：10.1016/j.ebiom.2024.105254 · 被引用次数：26 · 研究领域：Poxvirus research and outbreaks、Fungal Infections and Studies、Data-Driven Disease Surveillance

BACKGROUND: The dynamics of viral shedding and the specific humoral response against monkeypox virus (MPXV) have not been well characterized in patients across their disease course during hospitalisation. The aim of this study was to determine the viral load and the levels of antibodies against MPXV using longitudinal paired-collected samples from hospitalized patients. METHODS: Patients who were hospitalised with mpox were recruited at Beijing Ditan Hospital Capital Medical University in China between June 2 and September 23, 2023. Paired samples, including samples from skin lesions, the oropharynx, saliva, faeces, urine, plasma, and serum, were serially collected at days 1, 3, 7, and 14 after admission until discharge. Not all of the patients had samples obtained at all of the timepoints. All the samples were analysed via quantitative PCR. Virus isolation was performed by using clinical samples and Vero cells. The presence of IgM, IgA, IgG, and neutralising antibodies (NAbs) against MPXV was evaluated. The first collected plasma sample was taken when the patient was hospitalised, and the levels of cytokines and chemokines were measured in the sample. The demographic data, smallpox vaccination status, history of known exposure to MPVX, HIV status and other clinical data were collected using a standard case report form. FINDINGS: A total of 510 specimens were serially collected from 39 recruited people with mpox. Among all the samples, the skin lesions had the highest viral D...