New insights into the endothelial origin of hematopoietic system inspired by “TIF” approaches

作者：Siyuan Hou, Xia Guo, Junjie Du, Xiaochen Ding, Xiaowei Ning, Haizhen Wang, Haifeng Chen, Bing Liu, Yu Lan · 发表于：Blood Science · 年份：2024 · DOI：10.1097/bs9.0000000000000199 · 被引用次数：10 · 研究领域：Zebrafish Biomedical Research Applications、Single-cell and spatial transcriptomics、Immune cells in cancer

Hematopoietic stem progenitor cells (HSPCs) are derived from a specialized subset of endothelial cells named hemogenic endothelial cells (HECs) via a process of endothelial-to-hematopoietic transition during embryogenesis. Recently, with the usage of multiple single-cell technologies and advanced genetic lineage tracing techniques, namely, "TIF" approaches that combining transcriptome, immunophenotype and function/fate analyses, massive new insights have been achieved regarding the cellular and molecular evolution underlying the emergence of HSPCs from embryonic vascular beds. In this review, we focus on the most recent advances in the enrichment markers, functional characteristics, developmental paths, molecular controls, and the embryonic site-relevance of the key intermediate cell populations bridging embryonic vascular and hematopoietic systems, namely HECs and pre-hematopoietic stem cells, the immediate progenies of some HECs, in mouse and human embryos. Specifically, using expression analyses at both transcriptional and protein levels and especially efficient functional assays, we propose that the onset of Kit expression is at the HEC stage, which has previously been controversial.