Ribosome heterogeneity in development and disease

作者：Yuen Gao, Hongbing Wang · 发表于：Frontiers in Cell and Developmental Biology · 年份：2024 · DOI：10.3389/fcell.2024.1414269 · 被引用次数：8 · 研究领域：RNA modifications and cancer、RNA and protein synthesis mechanisms、Immune Cell Function and Interaction

Traditionally viewed as a fixed and homogeneous machinery for protein synthesis, the ribosome is increasingly recognized for its heterogeneity, as indicated by emerging studies highlighting the functional relevance of specialized ribosomes. However, whether ribosome heterogeneity is merely an outcome limited to specific conditions or a pervasive cellular phenomenon remains unclear, and existing evidence on the extensive existence of ribosome heterogeneity is scant. Here, we leveraged existing proteomic data and employed ribosome ratio-omics (Ribosome R ), which comprehensively analyzes ribosome protein stoichiometry across various biological samples exhibiting distinct functions, developmental stages, and pathological states. Using the 80S monosome proteomic data, Ribosome R analysis unveils significant ribosome heterogeneity across different tissues, including fat, spleen, liver, kidney, heart, and skeletal muscles. Furthermore, examination of testes at various stages of spermatogenesis reveals distinct Ribosome R signatures during tissue development. Analysis of the whole cell proteomic data finds that Ribosome R undergoes dynamic changes during in vitro neuronal maturation, indicating functional associations with specific molecular aspects of neurodevelopment. In pathological contexts, Ribosome R signatures in gastric tumors demonstrate functional links to pathways associated with tumorigenesis. Additionally, dynamic alterations in Ribosome R are observed in macrophages fo...