Development of a multi‐resistance and high‐yield rice variety using multigene transformation and gene editing

作者：Changyan Li, Zaihui Zhou, Xinzhu Xiong, Chuanxu Li, Chuanhong Li, Enlong Shen, Jianyu Wang, Wenjun Zha, Bian Wu, Hao Chen, Lei Zhou, Yongjun Lin, Aiqing You · 发表于：Plant Biotechnology Journal · 年份：2024 · DOI：10.1111/pbi.14434 · 被引用次数：18 · 研究领域：CRISPR and Genetic Engineering、GABA and Rice Research、Plant tissue culture and regeneration

Approximately one-third of the total annual food production in the world is lost owing to pests, diseases and weeds. Therefore, the challenges posed by crop losses and population growth have emphasized the need for better breeding techniques (FAO et al., 2023). Practical experience has demonstrated that the utilization of existing resistance genes to breed and cultivate herbicide- and pest-resistant rice cultivars is the most economical, safe and effective method for preventing and controlling weeds and pests (Zhang, 2007). The incorporation of a single or few resistance genes during rice breeding is no longer adequate for in-demand production. In addition, hybridization and backcrossing involve a long breeding cycle, and the issue of linkage drag may occur. The multi-gene transformation strategy can be utilized for the rapid and accurate incorporation of multiple resistance genes into rice (Zhu et al., 2017). The fact that a trade-off between growth and defence generally exists in crops is universally accepted. Therefore, the overexpression of multi-resistance genes in rice causes considerable changes to the agronomic traits of crops, especially yield. The crop yield is positively correlated with the flowering stage within a certain range. For example, editing Ehd1 or overexpressing Ghd7 to appropriately extend the basic vegetative growth period of rice may be possible, and ultimately promote rice yield and quality (Eshed and Lippman, 2019; Zhou et al., 2023). This strategy ...