Exploring the Health Benefits of Boletus aereus Polysaccharides: Extraction, Structural Characterization, and Antiproliferative Properties against Non-Hodgkin’s Lymphomas (NHLs)

作者：Haidi Wang, Linyu Yang, Qiang Qiu, Qianru Rao, Li Liu, Yuchen Cui, Liang Zhang, Yucheng Ma, Xi Jin, Dongxue Yang, Shiqian Qi, Kunjie Wang, Yong Li, Xufeng Zhang, Min Zhao · 发表于：Journal of Agricultural and Food Chemistry · 年份：2024 · DOI：10.1021/acs.jafc.4c03945 · 被引用次数：8 · 研究领域：Polysaccharides and Plant Cell Walls、Ginseng Biological Effects and Applications、Fungal Biology and Applications

Boletus aereus Fr. ex Bull. stands out as a delectable edible mushroom with high nutritional and medicinal values, featuring polysaccharides as its primary nutrient composition. In our continuous exploration of its beneficial substances, a novel polysaccharide (BAPN-1) with a molecular weight of 2279 kDa was prepared. It was identified as a glucan with a backbone composed of the residues →4)-α-Glc p -(1→ and →4,6)-α-Glc p -(1→ connected in a proportion of 5:1 and a β-Glc p -(1→ side residue attached at C6 of the →4,6)-α-Glc p -(1→ residue. Biologically, BAPN-1 exhibited broad-spectrum antiproliferative activities against various NHL cells, including HuT-78, OCI-LY1, OCI-LY18, Jurkat, RL, and Karpas-299, with IC 50 values of 0.73, 1.21, 3.18, 1.52, 3.34, and 4.25 mg/mL, respectively. Additionally, BAPN-1 significantly induced cell cycle arrest in the G2/M phase and caused apoptosis of NHL cells. Mechanistically, bulk RNA sequencing and Western blot analysis revealed that BAPN-1 could upregulate cyclin B1 and enhance cleaved caspase-9 expression through the inhibition of FGFR3 and RAF-MEK-ERK signaling pathways. This work supports the improved utilization of B. aereus in high-value health products.