Plasma proteomic comparisons change as coverage expands for SomaLogic and Olink

作者：Mary R. Rooney, Jingsha Chen, Christie M. Ballantyne, Ron C. Hoogeveen, Eric Boerwinkle, Bing Yu, Keenan A. Walker, Pascal Schlosser, Elizabeth Selvin, Nilanjan Chatterjee, David Couper, Morgan E. Grams, Josef Coresh · 发表于：medRxiv · 年份：2024 · DOI：10.1101/2024.07.11.24310161 · 被引用次数：35 · 研究领域：Advanced Proteomics Techniques and Applications、Advanced Biosensing Techniques and Applications、Single-cell and spatial transcriptomics

ABSTRACT The number of assays on highly-multiplexed proteomic platforms has grown ten-fold over the past 15 years from less than 1,000 to >11,000. The leading aptamer-based and antibody-based platforms have different strengths. For example, Eldjarn et al 1 demonstrated that the aptamer-based SomaScan 5k (4,907 assays, assessed in the Icelandic 36K) and the antibody-based Olink Explore 3072 (2,931 assays, assessed in the UK BioBank) had a similar number of cis -pQTLs among all targets (2,120 vs. 2,101) but Olink had a greater number of cis -pQTLs among the overlapping targets (1,164 vs. 1,467). Analysis of split plasma measures showed the SomaScan assays to be more precise: median coefficient of variation (CV) of 9.9% vs. 16.5% for Olink. 1 Precision of the newest versions of the platforms—SomaScan 11k (>11,000 assays, released in December 2023) and Olink Explore HT (>5,400 assays, released in July 2023)—has not yet been established. We assessed the reproducibility of the SomaScan 11k and Olink Explore HT using split plasma samples from 102 Atherosclerosis Risk in Communities (ARIC) Study participants. We found that the SomaScan 11k assays had a median CV of 6.8% (vs 6.6% for the subset of assays available on the SomaScan 5k) and the Olink Explore HT assays had a median CV of 35.7% (vs 19.8% for the subset of assays available on the Olink Explore 3072). Across Olink assays, the CVs were strongly negatively correlated with protein detectability, i.e., percent of sample...