Shrimp shapes a resistance trait against vibriosis by memorizing the colonization resistance of intestinal microbiota

作者：Jianbo Yuan, Yang Yu, Shihao Li, Xiaojun Zhang, Chuntao Zhang, Roujing Li, Jie Hu, Shuqing Si, Chengyi Zhang, Jianhai Xiang, Fuhua Li · 发表于：PLoS Pathogens · 年份：2024 · DOI：10.1371/journal.ppat.1012321 · 被引用次数：13 · 研究领域：Aquaculture disease management and microbiota、Vibrio bacteria research studies、Parasite Biology and Host Interactions

Vibriosis is one of the most serious diseases that commonly occurs in aquatic animals, thus, shaping a steady inherited resistance trait in organisms has received the highest priority in aquaculture. Whereas, the mechanisms underlying the development of such a resistance trait are mostly elusive. In this study, we constructed vibriosis-resistant and susceptible families of the Pacific white shrimp Litopenaeus vannamei after four generations of artificial selection. Microbiome sequencing indicated that shrimp can successfully develop a colonization resistance trait against Vibrio infections. This trait was characterized by a microbial community structure with specific enrichment of a single probiotic species (namely Shewanella algae), and notably, its formation was inheritable and might be memorized by host epigenetic remodeling. Regardless of the infection status, a group of genes was specifically activated in the resistant family through disruption of complete methylation. Specifically, hypo-methylation and hyper-expression of genes related to lactate dehydrogenase (LDH) and iron homeostasis might provide rich sources of specific carbon (lactate) and ions for the colonization of S. algae, which directly results in the reduction of Vibrio load in shrimp. Lactate feeding increased the survival of shrimp, while knockdown of LDH gene decreased the survival when shrimp was infected by Vibrio pathogens. In addition, treatment of shrimp with the methyltransferase inhibitor 5-azacyt...