Genetic Characteristics and Pathogenicity for Partial Gene fragment of one Bovine Rotavirus in Yunnan Province, China

作者：Chenxi Zhang, Zailei Li, Xinhui Duan, Hongya Yan, Xinyu Wu, Peiying Zhu, Wengui Li, Huafeng Gao · 发表于：Preprints.org · 年份：2024 · DOI：10.20944/preprints202407.0481.v1 · 被引用次数：1 · 研究领域：Viral gastroenteritis research and epidemiology、Congenital Anomalies and Fetal Surgery、Animal Virus Infections Studies

Group A rotaviruses (RVA) have been identified as the leading cause of neonatal calf diarrhea (NCD) In Yunnan, China, despite the limited documentation on this issue within the country. Given the significant impact on animal health and welfare, further attention is warranted to comprehend the prevalence and epidemiology of this disease. In this study, we isolated RVA strains from NCD clinical samples to support RVA epidemic study and further disease control in Yunnan. Material and Methods: Ten fecal samples were obtained from calves with a single episode of neonate calf diarrhea from a farmer during a severe NCD outbreak. For the diagnosis of Group A rotavirus (RVA) infection, fecal reverse transcription-polymerase chain reaction (RT-PCR) was employed as the primary method. Subsequently, RVA isolation was conducted on MA104 cells, with inoculates being pretreated with trypsin TPCK to enhance viral infectivity. The isolated RVA strain was further confirmed through RT-PCR and immunofluorescence assay. Results: RVA virus was isolated from clinical samples following six passages depended on trypsin treatment of inoculates. The sign of CPE detected consisted of increased cell granularity, obscure cell boundaries, cell rounding, and eventual degeneration and detachment of cells. Analysis of sequencing revealed that field isolates was of group A serotype. Phylogenetic analysis based on the ORF sequences of the VP4 and VP7 sequences segments indicated that the isolate was classified ...