Transcriptomic profiling of dynamic alternative splicing during the early response to Ralstonia solanacearum infection in tobacco roots

作者：Xiuming Wu, Ruimei Geng, Zhengwen Liu, Lirui Cheng, Caihong Jiang, Dan Liu, Aiguo Yang, Ying Tong, Daiwen Chen, Yangyang Sun, Zhiliang Xiao, Min Ren · 发表于：Plant Growth Regulation · 年份：2024 · DOI：10.1007/s10725-023-01112-y · 被引用次数：8 · 研究领域：Plant Pathogenic Bacteria Studies、Plant-Microbe Interactions and Immunity、Plant Disease Resistance and Genetics

Abstract The pathogenic bacterium, Ralstonia solanacearum , causes bacterial wilt disease in many crops, which leads to significant yield losses worldwide. Although genes associated with resistance to this pathogen have been isolated and characterized in crops, the molecular mechanisms underlying the plant–pathogen interactions remain to be elucidated. Here, we performed a comparative transcriptional profiling analysis of tobacco ( Nicotiana tabacum ) cultivars C048 (susceptible) and C244 (resistant) in response to R. solanacearum infection. We found that the number of down- and up-regulated genes increased dramatically 3 h post inoculation (hpi), peaked 24 hpi, and then decreased 48 and 72 hpi, representing a “transcriptomic shock”. Of these genes, those associated with biotic and abiotic stresses and secondary metabolism were up-regulated, whereas those associated with primary metabolism were down-regulated. Alternative splicing (AS) modulates root defense against R. solanacearum by fine-tuning gene expression during the transcriptomic responses to pathogen invasion. The numbers of skipped exon (SE) and mutually exclusive exon (MXE) type AS events were reduced by approximately 60–80% in roots 9–72 hpi compared to those occurring 0–3 hpi. On the contrary, the number of differential alternative splicing (DAS) events showing a change in isoform ratio between samples increased, and most of them were associated with the down-regulation of corresponding gene expression. In additi...