Trehalose along with ABA promotes the salt tolerance of Avicennia marina by regulating Na + transport

作者：Lingyu Song, Chaoqun Xu, Ludan Zhang, Jing Li, Liwei Jiang, Dongna Ma, Ze‐Jun Guo, Qian Wang, Xiuxiu Wang, Hai‐Lei Zheng · 发表于：The Plant Journal · 年份：2024 · DOI：10.1111/tpj.16921 · 被引用次数：19 · 研究领域：Plant Stress Responses and Tolerance、Plant Molecular Biology Research、Plant nutrient uptake and metabolism

SUMMARY Mangroves grow in tropical/subtropical intertidal habitats with extremely high salt tolerance. Trehalose and trehalose‐6‐phosphate (T6P) have an alleviating function against abiotic stress. However, the roles of trehalose in the salt tolerance of salt‐secreting mangrove Avicennia marina is not documented. Here, we found that trehalose was significantly accumulated in A. marina under salt treatment. Furthermore, exogenous trehalose can enhance salt tolerance by promoting the Na + efflux from leaf salt gland and root to reduce the Na + content in root and leaf. Subsequently, eighteen trehalose‐6‐phosphate synthase ( AmTPS ) and 11 trehalose‐6‐phosphate phosphatase ( AmTPP ) genes were identified from A. marina genome. Abscisic acid (ABA) responsive elements were predicted in AmTPS and AmTPP promoters by cis ‐acting elements analysis. We further identified AmTPS9A , as an important positive regulator, that increased the salt tolerance of AmTPS9A ‐overexpressing Arabidopsis thaliana by altering the expressions of ion transport genes and mediating Na + efflux from the roots of transgenic A. thaliana under NaCl treatments. In addition, we also found that ABA could promote the accumulation of trehalose, and the application of exogenous trehalose significantly promoted the biosynthesis of ABA in both roots and leaves of A. marina . Ultimately, we confirmed that AmABF2 directly binds to the AmTPS9A promoter in vitro and in vivo . Taken together, we speculated that there was a ...