Specificity of serological screening tests and reference laboratory tests to diagnose gambiense human African trypanosomiasis: a prospective clinical performance study

作者：Martial Kassi N’Djetchi, Oumou Camara, Mathurin Koffi, Mamadou Camara, Dramane Kaba, Jacques Kaboré, Alkali Tall, Brice Rotureau, Lucy Glover, Mélika Barkissa Traoré, Minayégninrin Koné, Bamoro Coulibaly, Guy Pacôme Adingra, Aïssata Soumah, M Gassama, Abdoulaye Dansy Camara, Charlie Franck Alfred Compaoré, Aïssata Camara, Salimatou Boiro, Elena Pérez Antón, Paul R. Bessell, Nick Van Reet, Bruno Bucheton, Vincent Jamonneau, Jean-Mathieu Bart, Philippe Solano, Sylvain Biéler, Veerle Lejon · 发表于：Infectious Diseases of Poverty · 年份：2024 · DOI：10.1186/s40249-024-01220-5 · 被引用次数：23 · 研究领域：Trypanosoma species research and implications、Malaria Research and Control、Parasites and Host Interactions

Abstract Background Serological screening tests play a crucial role to diagnose gambiense human African trypanosomiasis (gHAT). Presently, they preselect individuals for microscopic confirmation, but in future “screen and treat” strategies they will identify individuals for treatment. Variability in reported specificities, the development of new rapid diagnostic tests (RDT) and the hypothesis that malaria infection may decrease RDT specificity led us to evaluate the specificity of 5 gHAT screening tests. Methods During active screening, venous blood samples from 1095 individuals from Côte d’Ivoire and Guinea were tested consecutively with commercial (CATT, HAT Sero- K -SeT, Abbott Bioline HAT 2.0) and prototype (DCN HAT RDT, HAT Sero- K -SeT 2.0) gHAT screening tests and with a malaria RDT. Individuals with ≥ 1 positive gHAT screening test underwent microscopy and further immunological (trypanolysis with T.b. gambiense LiTat 1.3, 1.5 and 1.6; indirect ELISA/ T.b. gambiense ; T.b. gambiense inhibition ELISA with T.b. gambiense LiTat 1.3 and 1.5 VSG) and molecular reference laboratory tests (PCR TBRN3, 18S and TgsGP; SHERLOCK 18S Tids, 7SL Zoon , and TgsGP; Trypanozoon S 2 -RT-qPCR 18S2, 177T, GPI-PLC and TgsGP in multiplex ; RT-qPCR DT8, DT9 and TgsGP in multiplex). Microscopic trypanosome detection confirmed gHAT, while other individuals were considered gHAT free. Differences in fractions between groups were assessed by Chi square and differences in specificity between 2 test...