Comprehensive identification of plant peroxisome targeting signal type 1 tripeptides

作者：Qianwen Deng, Xiao Hong, Yuqing Xia, Zhicheng Gong, Huaxin Dai, Jiarong Chen, Yanlei Feng, Jianfeng Zhang, Xiaodong Xie, Nannan Li, Xingxing Shen, Jianping Hu, Qiang Zhang, Xuye Lang, Ronghui Pan · 发表于：New Phytologist · 年份：2024 · DOI：10.1111/nph.19955 · 被引用次数：9 · 研究领域：Peroxisome Proliferator-Activated Receptors、Metabolism, Diabetes, and Cancer、RNA modifications and cancer

Peroxisomes are universal eukaryotic organelles hosting various metabolic pathways, with particularly diverse metabolic roles in plants (Pan et al., 2020). Severe peroxisomal dysfunction can lead to fatal genetic disorders in humans and embryonic lethality in plants (Hu et al., 2012; Honsho et al., 2020). The proteome and metabolism of peroxisomes vary significantly between species, organs, and developmental stages, and in response to different environmental conditions (Gabaldón, 2018; Pan & Hu, 2018; Yifrach et al., 2018; Corpas, 2019). Peroxisomes not only have shared but also species-specific functions (Sibirny, 2016; Kao et al., 2018; Pan & Hu, 2018; Pan et al., 2020). Mass spectrometry (MS)-based peroxisome proteomics studies in different Arabidopsis developmental stages and tissue types, such as etiolated seedlings, green leaves, senescent leaves, and cultured cells (Fukao et al., 2002, 2003; Reumann et al., 2007, 2009; Eubel et al., 2008; Quan et al., 2013; Pan et al., 2018; Pan & Hu, 2018), as well as in several other plant species, such as soybean, spinach, sweet pepper, and castor bean (Arai et al., 2008; Babujee et al., 2010; González-Gordo et al., 2022; Wrobel et al., 2023), have uncovered hundreds of peroxisomal proteins and significantly improved our understanding of these metabolically plastic organelles. Another powerful strategy in uncovering peroxisome functions is in silico identification of proteins containing peroxisome targeting signals (PTSs), which req...