The protective effects of aqueous extract of Schisandra sphenanthera against alcoholic liver disease partly through the PI3K-AKT-IKK signaling pathway

作者：Ding Liu, Kai Yang, Taotao Li, Tiantian Tang, Yujiao Wang, Wenfei Wang, Jia Li, Peijie Zhou, Xuan Wang, Chongbo Zhao, Dongyan Guo, Yundong Xie, Jiangxue Cheng, Mei Wang, Jing Sun, Xiaofei Zhang · 发表于：Heliyon · 年份：2024 · DOI：10.1016/j.heliyon.2024.e34214 · 被引用次数：8 · 研究领域：Alcohol Consumption and Health Effects、Plant-derived Lignans Synthesis and Bioactivity、Drug-Induced Hepatotoxicity and Protection

PurposeThis study aimed to investigated the key chemical components and the effect of the aqueous extract of Schisandra sphenanthera (SSAE) on alcoholic liver disease (ALD) and the related molecular mechanism.MethodsThis study employed UPLC-Q-TOF-MS/MS to identify the chemical compositions in SSAE. ALD rat model was established through oral administration of white spirit. Transcriptome sequencing, weighted gene co-expression network construction analysis (WGCNA), and network pharmacology were used to predict key compositions and pathways targeted by SSAE for the treatment of ALD. Enzyme-linked immunosorbent assay (ELISA), biochemical kits, hematoxylin-eosin (HE) staining, Western blotting (WB) analysis, and immunohistochemical analysis were used to validate the mechanism of action of SSAE in treating ALD.ResultsActive ingredients such as schisandrin A, schisandrol A, and schisandrol B were found to regulate the PI3K/AKT/IKK signaling pathway. Compared to the model group, the SSAE group demonstrated significant improvements in cellular solidification and tissue inflammation in the liver tissues of ALD model rats. Additionally, SSAE regulated the levels of a spartate aminotransferase (AST), alanine aminotransferase (ALT), alcohol dehydrogenase (ADH), and aldehyde Dehydrogenase (ALDH) in serum (P < 0.05); Western blotting and immunohistochemical analyses showed that the expression levels of phosphorylated PI3K, AKT, IKK, NFκB, and FOXO1 proteins were significantly reduced in liv...