Single‐cell sequencing: Current applications in various tuberculosis specimen types

作者：Yuqin Zeng, Quan Ma, Jinyun Chen, Xingxing Kong, Zhanpeng Chen, Huazhen Liu, Lanlan Liu, Qian Yan, Xiaomin Wang, Shuihua Lu · 发表于：Cell Proliferation · 年份：2024 · DOI：10.1111/cpr.13698 · 被引用次数：7 · 研究领域：Single-cell and spatial transcriptomics、Mycobacterium research and diagnosis、Tuberculosis Research and Epidemiology

Tuberculosis (TB) is a chronic disease caused by Mycobacterium tuberculosis (M.tb) and responsible for millions of deaths worldwide each year. It has a complex pathogenesis that primarily affects the lungs but can also impact systemic organs. In recent years, single-cell sequencing technology has been utilized to characterize the composition and proportion of immune cell subpopulations associated with the pathogenesis of TB disease since it has a high resolution that surpasses conventional techniques. This paper reviews the current use of single-cell sequencing technologies in TB research and their application in analysing specimens from various sources of TB, primarily peripheral blood and lung specimens. The focus is on how these technologies can reveal dynamic changes in immune cell subpopulations, genes and proteins during disease progression after M.tb infection. Based on the current findings, single-cell sequencing has significant potential clinical value in the field of TB research. Next, we will focus on the real-world applications of the potential targets identified through single-cell sequencing for diagnostics, therapeutics and the development of effective vaccines.