Neutrophil extracellular traps-inspired DNA hydrogel for wound hemostatic adjuvant

作者：Rui Ye, Ziyu Zhu, Tianyi Gu, Dengjie Cao, Kai Jiang, Qiang Dai, Kuoran Xing, Yifan Jiang, Siyi Zhou, Ping Cai, David Tai Leong, Mengfei Yu, Jie Song · 发表于：Nature Communications · 年份：2024 · DOI：10.1038/s41467-024-49933-3 · 被引用次数：95 · 研究领域：Hemostasis and retained surgical items、Wound Healing and Treatments、Surgical Sutures and Adhesives

Severe traumatic bleeding may lead to extremely high mortality rates, and early intervention to stop bleeding plays as a critical role in saving lives. However, rapid hemostasis in deep non-compressible trauma using a highly water-absorbent hydrogel, combined with strong tissue adhesion and bionic procoagulant mechanism, remains a challenge. In this study, a DNA hydrogel (DNAgel) network composed of natural nucleic acids with rapid water absorption, high swelling and instant tissue adhesion is reported, like a band-aid to physically stop bleeding. The excellent swelling behavior and robust mechanical performance, meanwhile, enable the DNAgel band-aid to fill the defect cavity and exert pressure on the bleeding vessels, thereby achieving compression hemostasis for deep tissue bleeding sites. The neutrophil extracellular traps (NETs)-inspired DNAgel network also acts as an artificial DNA scaffold for erythrocytes to adhere and aggregate, and activates platelets, promoting coagulation cascade in a bionic way. The DNAgel achieves lower blood loss than commercial gelatin sponge (GS) in male rat trauma models. In vivo evaluation in a full-thickness skin incision model also demonstrates the ability of DNAgel for promoting wound healing. Overall, the DNAgel band-aid with great hemostatic capacity is a promising candidate for rapid hemostasis and wound healing.