Photoelectrochemical Solution Gated Graphene Field‐Effect Transistor Functionalized by Enzymatic Cascade Reaction for Organophosphate Detection

作者：Hairui Wang, Enhui Hou, Na Xu, Yu‐Feng Zhang, Jianfeng Wu, Weijian Yuan, Zhi‐Guo Kong, Ping Nie, Limin Chang, Xuelin Zhang, Jianwei Xie · 发表于：Small · 年份：2024 · DOI：10.1002/smll.202402655 · 被引用次数：19 · 研究领域：Advanced biosensing and bioanalysis techniques、Electrochemical sensors and biosensors、Conducting polymers and applications

Solution Gated Graphene Field-Effect Transistors (SGGT) are eagerly anticipated as an amplification platform for fabricating advanced ultra-sensitive sensors, allowing significant modulation of the drain current with minimal gate voltage. However, few studies have focused on light-matter interplay gating control for SGGT. Herein, this challenge is addressed by creating an innovative photoelectrochemical solution-gated graphene field-effect transistor (PEC-SGGT) functionalized with enzyme cascade reactions (ECR) for Organophosphorus (OPs) detection. The ECR system, consisting of acetylcholinesterase (AChE) and CuBTC nanomimetic enzymes, selectively recognizes OPs and forms o-phenylenediamine (oPD) oligomers sediment on the PEC electrode, with layer thickness related to the OPs concentration, demonstrating time-integrated amplification. Under light stimulation, the additional photovoltage generated on the PEC gate electrode is influenced by the oPD oligomers sediment layer, creating a differentiated voltage distribution along the gate path. PEC-SGGT, inherently equipped with built-in amplification circuits, sensitively captures gate voltage changes and delivers output with an impressive thousandfold current gain. The seamless integration of these three amplification modes in this advanced sensor allows a good linear range and highly sensitive detection of OPs, with a detection limit as low as 0.05 pm. This work provides a proof-of-concept for the feasibility of light-assisted f...