Recent advances in identifying protein targets of bioactive natural products

作者：Xuan Jiang, Kinyu Shon, Xiaofeng Li, Xiaofeng Li, Guoliang Cui, Yuanyuan Wu, Zhonghong Wei, Aiyun Wang, Yin Lu, Xiaoman Li, Yin Lu · 发表于：Heliyon · 年份：2024 · DOI：10.1016/j.heliyon.2024.e33917 · 被引用次数：10 · 研究领域：Click Chemistry and Applications、Biochemical and Structural Characterization、Chemical Synthesis and Analysis

Background: Natural products exhibit structural complexity, diversity, and historical therapeutic significance, boasting attractive functions and biological activities that have significantly influenced drug discovery endeavors. The identification of target proteins of active natural compounds is crucial for advancing novel drug innovation. Currently, methods for identifying targets of natural products can be categorized into labeling and label-free approaches based on whether the natural bioactive constituents are modified into active probes. In addition, there is a new avenue for rapidly exploring the targets of natural products based on their innate functions. Aim: This review aimed to summarize recent advancements in both labeling and label-free approaches to the identification of targets for natural products, as well as the novel target identification method based on the natural functions of natural products. Methods: We systematically collected relevant articles published in recent years from PubMed, Web of Science, and ScienceDirect, focusing on methods employed for identifying protein targets of bioactive natural products. Furthermore, we systematically summarized the principles, procedures, and successful cases, as well as the advantages and limitations of each method. Results: Labeling methods allow for the direct labeling of target proteins and the exclusion of indirectly targeted proteins. However, these methods are not suitable for studying post-modified compound...