Palladium/Platinum/Ruthenium Trimetallic Dendritic Nanozymes Exhibiting Enhanced Peroxidase-like Activity for Signal Amplification of Lateral Flow Immunoassays

作者：Weiguo Wang, Qianqian Cao, Jian He, Yafeng Xie, Ying Zhang, Lin Yang, Minghui Duan, Jikai Wang, Wei Li · 发表于：Nano Letters · 年份：2024 · DOI：10.1021/acs.nanolett.4c01568 · 被引用次数：30 · 研究领域：Advanced Nanomaterials in Catalysis、Electrochemical sensors and biosensors、Neuroinflammation and Neurodegeneration Mechanisms

Developing ultrasensitive lateral flow immunoassays (LFIAs) has garnered significant attention in the field of point-of-care testing. In this study, a trimetallic dendritic nanozyme (Pd@Pt–Ru) was synthesized through Ru deposition on a Pd@Pt core and utilized to enhancing the sensitivity of LFIAs. Pd@Pt–Ru exhibited a K m value of 5.23 mM for detecting H 2 O 2, which indicates an H 2 O 2 affinity comparable with that of horseradish peroxidase. The Ru surface layer reduces the activation energy barrier, which increases the maximum reaction rate. As a proof of concept, the proposed Pd@Pt–Ru nanozyme was incorporated into LFIAs (A–Pd@Pt–Ru–LFIAs) for detecting human chorionic gonadotropin (hCG). Compared with conventional gold nanoparticle (AuNP)–LFIAs, A–Pd@Pt–Ru–LFIAs demonstrated 250-fold increased sensitivity, thereby enabling a visible detection limit as low as 0.1 IU/L. True positive and negative rates both reached 100%, which renders the proposed Pd@Pt–Ru nanozyme suitable for detecting hCG in clinical samples.