Transcriptome‐wide N 6 ‐methyladenosine profiling reveals growth–defense trade‐offs in the response of rice to brown planthopper ( Nilaparvata lugens ) infestation

作者：Shuai Li, Xinyang Tan, Zhen He, Lei Jiang, Yali Li, Yang Liu, Ary A. Hoffmann, Chunqing Zhao, Jichao Fang, Rui Ji · 发表于：Pest Management Science · 年份：2024 · DOI：10.1002/ps.8265 · 被引用次数：9 · 研究领域：RNA modifications and cancer、Plant Disease Resistance and Genetics、HVDC Systems and Fault Protection

Abstract BACKGROUND N 6 ‐Methyladenosine (m 6 A) is a common messenger RNA (mRNA) modification that affects various physiological processes in stress responses. However, the role of m 6 A modifications in plants responses to herbivore stress remains unclear. RESULTS Here, we found that an infestation of brown planthopper ( Nilaparvata lugens ) female adults enhanced the resistance of rice to N. lugens . The m 6 A methylome analysis of N. lugens ‐infested and uninfested rice samples was performed to explore the interaction between rice and N. lugens . The m 6 A methylation mainly occurred in genes that were actively expressed in rice following N. lugens infestation, while an analysis of the whole‐genomic mRNA distribution of m 6 A showed that N. lugens infestation caused an overall decrease in the number of m 6 A methylation sites across the chromosomes. The m 6 A methylation of genes involved in the m 6 A modification machinery and several defense‐related phytohormones (jasmonic acid and salicylic acid) pathways was increased in N. lugens ‐infested rice compared to that in uninfested rice. In contrast, m 6 A modification levels of growth‐related phytohormone (auxin and gibberellin) biosynthesis‐related genes were significantly attenuated during N. lugens infestation, accompanied by the down‐regulated expression of these transcripts, indicating that rice growth was restricted during N. lugens attack to rapidly optimize resource allocation for plant defense. Integrative analysi...