Utility of leukocyte‐associated immunoglobulin‐like receptor‐1 ( CD305 ) in flow cytometric detection of minimal bone marrow involvement by B ‐cell non‐ Hodgkin lymphoma

作者：Anu Singh, Jagruti Patil, Sitaram Ghogale, Nilesh Umakant Deshpande, Karishma Girase, Navami Shetye, Sweta Rajpal, Gaurav Chatterjee, Nikhil V. Patkar, Disha Jain, Sridhar Epari, Tanuja M. Shet, Sumeet Gujral, Papagudi Ganesan Subramanian, Prashant Ramesh Tembhare · 发表于：Cytometry Part B Clinical Cytometry · 年份：2024 · DOI：10.1002/cyto.b.22193 · 被引用次数：4 · 研究领域：Lymphoma Diagnosis and Treatment、Chronic Lymphocytic Leukemia Research、CNS Lymphoma Diagnosis and Treatment

Multicolor flow cytometry (MFC) is crucial in detecting occult or minimal bone marrow (BM) involvement by non-Hodgkin lymphomas (NHL), which may not be detected using trephine biopsy or imaging studies. Detection of low-level BM involvement can be challenging without definite immunophenotypic aberrancies. We studied the utility of CD305 in MFC detection of minimal BM involvement by B-NHL, especially in the absence of aberrancies by commonly used markers. The study included 1084 consecutive BM samples submitted for the staging of B-NHLs (excluding CLL) over two years. Samples were studied for morphological, immunophenotypic, and histopathological assessment. MFC studies were performed using 10-13 color MFC, including CD305-antibody (clone, DX26). Minimal BM involvement was defined with a cutoff of ≤10% lymphoma cells in viable cells on MFC assessment. Of 1084, 148 samples revealed overt morphological involvement by B-NHL and were excluded from analysis. BM samples of 172/936 patients were morphologically negative but revealed involvement using MFC independently. Corresponding trephine biopsy involvement was detected in only 79/172 (45.9%) patients. On MFC, 23/172 samples showed BM involvement with >10% lymphoma cells, and 149/172 (86.6%) samples revealed minimal involvement. In 54/149 (36.24%) samples, lymphoma cells were detected only with aberrant loss of CD305 expression. In 78 of the remaining 95 samples (82.1%), it provided an immunophenotypic aberrancy addition to other ...