Genome resequencing reveals the genetic basis of population evolution, local adaptation, and rewiring of the rhizome metabolome in Atractylodes lancea

作者：Chengcai Zhang, Sheng Wang, Jiahui Sun, Xiangkong Li, Hongyang Wang, Xiuzhi Guo, Yuefeng Wang, Daiquan Jiang, Chaogeng Lyu, Chuanzhi Kang, Yan Zhang, Zengxu Xiang, Qingjun Yuan, Binbin Yan, Ming Qin, Luqi Huang, Lanping Guo · 发表于：Horticulture Research · 年份：2024 · DOI：10.1093/hr/uhae167 · 被引用次数：25 · 研究领域：Traditional Chinese Medicine Analysis、Natural product bioactivities and synthesis、Ginseng Biological Effects and Applications

Abstract The formation of high-quality Chinese medicinal materials is a micro-evolutionary process of multiple genes involving quantitative inheritance under environmental stress. Atractylodes lancea is a traditionally used medicinal plant in China that is broadly distributed and possesses a considerable amount of essential oils. However, to date, limited research has been conducted to characterize the genetics and metabolites of A. lancea shaped by natural variation. Hence, we assembled a high-quality genome of A. lancea, featuring a contig N50 of 1.18 Mb. We further integrated population resequencing of A. lancea and conducted analyses to characterize its genetic diversity, population evolution, and rewiring of volatile metabolites. The natural variation effect exerted significant pressure on A. lancea from different geographic locations, resulting in genetic differentiation among three groups. Correlation analysis of metabolites in A. lancea revealed significant natural variations of terpenoids, heterocyclic compounds, ketones, and esters. We also found that 427 metabolites displayed noteworthy divergence due to directional selection. Additionally, our genome-wide association studies on the metabolome for medicinal quality traits identified several candidate genes, such as AlZFP706 and AlAAHY1, exhibiting significant correlations with atractylodin and hinesol levels, respectively. Overall, this study provides an intricate genomic resource for A. lancea, thereby expanding o...