Tobacco crop rotation enhances the stability and complexity of microbial networks

作者：Huilin Yan, Shaolong Wu, Ping Li, Xin Jin, Dejun Shi, Danjia Tu, Weiai Zeng, Lin Tan · 发表于：Frontiers in Microbiology · 年份：2024 · DOI：10.3389/fmicb.2024.1416256 · 被引用次数：15 · 研究领域：Soil Carbon and Nitrogen Dynamics、Microbial Community Ecology and Physiology、Mycorrhizal Fungi and Plant Interactions

Introduction The effects of continuous cropping and rotation cropping, two important tobacco cultivation practices, on soil microbial communities at different stages remain unclear. Different planting patterns have been shown to influence soil physical and chemical properties, which in turn can affect the composition and diversity of soil microbial communities. Methods In order to investigate the impact of different planting methods on soil microbial community structure, we selected two representative planting methods: continuous cropping (tobacco) and rotational cropping (tobacco-maize). These methods were chosen as the focal points of our research to explore the potential effects on soil microbial communities. High-throughput sequencing technology was employed to investigate the structure of soil microbial communities, as well as their relationships with soil environmental factors, by utilizing the 16S rRNA, ITS, and 18S genes. Furthermore, the interaction among microorganisms was explored through the application of the Random Matrix Theory (RMT) molecular ecological network approach. Results There was no significant difference in α diversity, but significant difference in β diversity based on Jaccard distance test. Compared to continuous cropping, crop rotation significantly increased the abundance of beneficial prokaryotes Verrucomicrobia and Rhodanobacter. These findings indicate that crop rotation promotes the enrichment of Verrucomicrobia and Rhodanobacter in the soil ...