Comprehensive chemical profiling with UHPLC-MS, in-vitro, in-silico, and in-vivo antidiabetic potential of Typha domingensis Pers; A novel source of bioactive compounds

作者：Rizwana Dilshad, Kashif-ur-Rehman Khan, Rihana Dilshad, Saeed Ahmad, Huma Rao, Umair Khurshid, Sultan Ahmad, Maqsood Ahmad, Hafiz Muhammad Usman Abid, Muhammad Kashif Zaman, Rabia Nisar, Saharish Khaliq, Bilal Ahmad Ghalloo · 发表于：South African Journal of Botany · 年份：2024 · DOI：10.1016/j.sajb.2024.06.007 · 被引用次数：13 · 研究领域：Natural Antidiabetic Agents Studies、Medicinal Plants and Bioactive Compounds、Biological and pharmacological studies of plants

Aim of study Typha is one of the valuable plant genera in folklore use in diabetes, and it can be a good source of bioactive chemicals. The current project was planned to investigate the pharmaceutical potential of n -butanol fraction of Typha domingensis Pers. (TDBF), a less explored medicinal plant. Materials and methods The phytochemical composition of the TDBF was assessed through a comprehensive analysis, encompassing the quantification of total bioactive contents (including total tannins and HPLC-PDA quantification) and a thorough exploration of secondary metabolites through scanning with UHPLC-MS. The pharmacological assessment included the determination of antioxidant activity (Total Antioxidant Capacity and Metal Chelating Assay), hemolytic potential, in-vitro antidiabetic effects (α-glucosidase and α-amylase inhibition, and antiglycation activity), as well as in-vivo antidiabetic activities . Molecular docking and ADMET studies were also performed for UHPLC-MS identified compounds. Results The TDBF showed 134.76 ± 8.23 mg TAE/g total tannin contents. With HPLC-PDA, p -Hydroxy benzoic acid and p -Coumaric acid were quantified. During UHPLC-MS scanning, a total of five bioactive metabolites were identified in the negative mode, while 54 were detected in the positive mode. The in-vivo assessment of antidiabetic activity revealed a notable reduction in blood glucose levels in diabetic rats following treatment with the extract, demonstrating its efficacy compared to the ...