Characteristics of the gut microbiota and serum metabolites in postmenopausal women with reduced bone mineral density

作者：Litao Yan, Xianfeng Wang, Tiantian Yu, Zhiming Qi, Huan Li, Nan Hao, Kun Wang, Di Luo, Hua Fei, Wendong Wang · 发表于：Frontiers in Cellular and Infection Microbiology · 年份：2024 · DOI：10.3389/fcimb.2024.1367325 · 被引用次数：29 · 研究领域：Gut microbiota and health、Metabolomics and Mass Spectrometry Studies、Bone health and osteoporosis research

Introduction Emerging evidence suggests that the gut microbiota is closely associated with bone homeostasis. However, little is known about the relationships among the bone mineral density (BMD) index, bone turnover markers, and the gut microbiota and its metabolites in postmenopausal women. Methods In this study, to understand gut microbiota signatures and serum metabolite changes in postmenopausal women with reduced BMD, postmenopausal individuals with normal or reduced BMD were recruited and divided into normal and OS groups. Feces and serum samples were collected for 16S rRNA gene sequencing, liquid chromatography coupled with mass spectrometry (LC-MS)-based metabolomics and integrated analysis. Results The results demonstrated that bacterial richness and diversity were greater in the OS group than in the normal group. Additionally, distinguishing bacteria were found among the two groups and were closely associated with the BMD index and bone turnover markers. Metabolomic analysis revealed that the expression of serum metabolites, such as etiocholanolone, testosterone sulfate, and indole-3-pyruvic acid, and the corresponding signaling pathways, especially those involved in tryptophan metabolism, fatty acid degradation and steroid hormone biosynthesis, also changed significantly. Correlation analysis revealed positive associations between normal group-enriched Bacteroides abundance and normal group-enriched etiocholanolone and testosterone sulfate abundances; in particular...