Large-field objective lens for multi-wavelength microscopy at mesoscale and submicron resolution

作者：Xin Xu, Qin Luo, Jixiang Wang, Yahui Song, Hong Ye, Xin Zhang, Y. He, Minxuan Sun, Ruobing Zhang, Guohua Shi · 发表于：Opto-Electronic Advances · 年份：2024 · DOI：10.29026/oea.2024.230212 · 被引用次数：70 · 研究领域：Advanced Fluorescence Microscopy Techniques、Near-Field Optical Microscopy、Advanced Electron Microscopy Techniques and Applications

Conventional microscopes designed for submicron resolution in biological research are hindered by a limited field of view, typically around 1 mm. This restriction poses a challenge when attempting to simultaneously analyze various parts of a sample, such as different brain areas. In addition, conventional objective lenses struggle to perform consistently across the required range of wavelengths for brain imaging in vivo . Here we present a novel mesoscopic objective lens with an impressive field of view of 8 mm, a numerical aperture of 0.5, and a working wavelength range from 400 to 1000 nm. We achieved a resolution of 0.74 μm in fluorescent beads imaging. The versatility of this lens was further demonstrated through high-quality images of mouse brain and kidney sections in a wide-field imaging system, a confocal laser scanning system, and a two-photon imaging system. This mesoscopic objective lens holds immense promise for advancing multi-wavelength imaging of large fields of view at high resolution.