QTL analysis on a lemon population provides novel insights on the genetic regulation of the tolerance to the two-spotted spider mite attack

作者：C. Catalano, Mario Di Guardo, G. Licciardello, S. Seminara, Giovanna Tropea Garzia, Antonio Biondi, Michela Troggio, Luca Bianco, Stefano La Malfa, Alessandra Gentile, Gaetano Distefano · 发表于：BMC Plant Biology · 年份：2024 · DOI：10.1186/s12870-024-05211-4 · 被引用次数：6 · 研究领域：Genetic Mapping and Diversity in Plants and Animals、Insect-Plant Interactions and Control、Phytoplasmas and Hemiptera pathogens

BACKGROUND: Among the Citrus species, lemon (Citrus limon Burm f.) is one of the most affected by the two-spotted spider mite (Tetranychus urticae Koch). Moreover, chemical control is hampered by the mite's ability to develop genetic resistance against acaricides. In this context, the identification of the genetic basis of the host resistance could represent a sustainable strategy for spider mite control. In the present study, a marker-trait association analysis was performed on a lemon population employing an association mapping approach. An inter-specific full-sib population composed of 109 accessions was phenotyped through a detached-leaf assays performed in modified Huffaker cells. Those individuals, complemented with two inter-specific segregating populations, were genotyped using a target-sequencing approach called SPET (Single Primer Enrichment Technology), the resulting SNPs were employed for the generation of an integrated genetic map. RESULTS: The percentage of damaged area in the full-sib population showed a quantitative distribution with values ranging from 0.36 to 9.67%. A total of 47,298 SNPs were selected for an association mapping study and a significant marker linked with resistance to spider mite was detected on linkage group 5. In silico gene annotation of the QTL interval enabled the detection of 13 genes involved in immune response to biotic and abiotic stress. Gene expression analysis showed an over expression of the gene encoding for the ethylene-respon...