AutoESDCas: A Web-Based Tool for the Whole-Workflow Editing Sequence Design for Microbial Genome Editing Based on the CRISPR/Cas System

作者：Chunhe Yang, Yi Yang, Guangyun Chu, Ruoyu Wang, Haoran Li, Yufeng Mao, Meng Wang, Jian Zhang, Xiao‐Ping Liao, Hongwu Ma · 发表于：ACS Synthetic Biology · 年份：2024 · DOI：10.1021/acssynbio.4c00063 · 被引用次数：4 · 研究领域：CRISPR and Genetic Engineering、RNA and protein synthesis mechanisms、Bacterial Genetics and Biotechnology

Genome editing is the basis for the modification of engineered microbes. In the process of genome editing, the design of editing sequences, such as primers and sgRNA, is very important for the accurate positioning of editing sites and efficient sequence editing. The whole process of genome editing involves multiple rounds and types of editing sequence design, while the development of related whole-workflow design tools for high-throughput experimental requirements lags. Here, we propose AutoESDCas, an online tool for the end-to-end editing sequence design for microbial genome editing based on the CRISPR/Cas system. This tool facilitates all types of genetic manipulation covering diverse experimental requirements and design scenarios, enables biologists to quickly and efficiently obtain all editing sequences needed for the entire genome editing process, and empowers high-throughput strain modification. Notably, with its off-target risk assessment function for editing sequences, the usability of the design results is significantly improved. AutoESDCas is freely available at https://autoesdcas.biodesign.ac.cn/with the source code at https://github.com/tibbdc/AutoESDCas/.