Electrospun radially oriented berberine-PHBV nanofiber dressing patches for accelerating diabetic wound healing

作者：Qiuyu Wang, Sai Zhang, Jiayi Jiang, Shaojuan Chen, Seeram Ramakrishna, Wenwen Zhao, Fan Yang, Shaohua Wu · 发表于：Regenerative Biomaterials · 年份：2024 · DOI：10.1093/rb/rbae063 · 被引用次数：55 · 研究领域：Electrospun Nanofibers in Biomedical Applications、Nerve injury and regeneration、Wound Healing and Treatments

Abstract A dressing patch made of radially oriented poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers was successfully manufactured with a modified electrospinning strategy. The as-electrospun PHBV radially oriented nanofiber dressing patch exhibited uniform and bead-free nanofibrous morphology and innovative radially oriented arrangement, which was demonstrated to possess obviously improved mechanical property, increased surface hydrophilicity and enhanced biological properties compared to the PHBV nanofiber dressing patch control with traditionally randomly oriented pattern. Interestingly, it was found that the radially oriented pattern could induce the cell migration from the periphery to the center along the radially oriented nanofibers in a rapid manner. To further improve the biofunction of PHBV radially oriented nanofiber dressing patch, berberine (Beri, an isoquinoline alkaloid) with two different concentrations were encapsulated into PHBV nanofibers during electrospinning, which were found to present a sustained drug release behavior for nearly one month. Importantly, the addition of Beri could impart the dressing patch with excellent anti-inflammatory property by significantly inhibiting the secretion of pro-inflammatory factors of M1 macrophages, and also showed an additive influence on promoting the proliferation of human dermal fibroblasts (HDFs), as well as inhibiting the growth of E. coli, S. aureus and C. albicans, compared with the Beri-free dres...