A novel glycoglycerolipid from Holotrichia diomphalia Bates: Structure characteristics and protective effect against DNA damage

作者：Wenjuan Liu, Yu-He Qiao, Shuyao Wang, Yubo Wang, Qiu-Na Nong, Qianhan Xiao, Hong-Xin Bai, Kehan Wu, Jie Chen, Xiaoqiang Li, Yufan Wang, Jin Tan, Wei Cao · 发表于：International Journal of Biological Macromolecules · 年份：2024 · DOI：10.1016/j.ijbiomac.2024.132594 · 被引用次数：4 · 研究领域：Plant Parasitism and Resistance、Bryophyte Studies and Records、Insect Utilization and Effects

A lipidated polysaccharide , HDPS-2II, was isolated from the dried larva of Holotrichia diomphalia , which is used in traditional Chinese medicine . The molecular weight of HDPS-2II was 5.9 kDa, which contained a polysaccharide backbone of →4)-β-Man p -(1 → 4,6)-β-Man p -(1 → [6)-α-Glc p -(1] n → 6)-α-Glc p → with the side chain α-Glc p -(6 → 1)-α-Glc p -(6 → linked to the C-4 of β-1,4,6-Man p and four types of lipid chains including 4-(4-methyl-2-(methylamino)pentanamido)pentanoic acid, 5-(3-(tert-butyl)phenoxy)hexan-2-ol, N-(3-methyl-5-oxopentan-2-yl)palmitamide, and N-(5-amino-3-methyl-5-oxopentan-2-yl)stearamide. The lipid chains were linked to C-1 of terminal α-1,6-Glc p in carbohydrate chain through diacyl-glycerol. HDPS-2II exhibited DNA protective effects and antioxidative activity on H 2 O 2 - or adriamycin (ADM)-induced Chinese hamster lung cells. Furthermore, HDPS-2II significantly ameliorated chromosome aberrations and the accumulation of reactive oxygen species (ROS), reduced γ-H2AX signaling and the expressions of NADPH oxidase (NOX)2, NOX4, P22 phox , and P47 phox in ADM-induced cardiomyocytes. Mechanistically, HDPS-2II suppressed ADM-induced up-regulation of NOX2 and NOX4 in cardiomyocytes, but not in NOX2 or NOX4 knocked-down cardiomyocytes, indicating that HDPS-2II could relieve intracellular DNA damage by regulating NOX2/NOX4 signaling. These findings demonstrate that HDPS-2II is a new potential DNA protective agent.