Qizhu anticancer prescription enhances immunosurveillance of liver cancer cells by regulating p21-dependent secretory phenotypes

作者：Rui Hu, Jing Li, Qi Huang, Xin Zhong, Jialing Sun, Jinyu Yi, Lanfen Peng, Xinning Liu, Yuan Yang, Wenmin Yang, Yan Wang, Wenfeng Ma, Wenxing Feng, Youhua Xu, Xiaozhou Zhou · 发表于：Journal of Ethnopharmacology · 年份：2024 · DOI：10.1016/j.jep.2024.118400 · 被引用次数：10 · 研究领域：Telomeres, Telomerase, and Senescence、Cancer-related Molecular Pathways、Retinoids in leukemia and cellular processes

Ethnopharmacological relevance Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide, largely due to the limitations of available therapeutic strategies. The traditional Chinese medicine Qizhu Anticancer Prescription (QZACP) can improve the quality of life and prolong the survival time of patients with HCC. However, the precise mechanisms underlying the anti-cancer properties of QZACP remain unclear. Purpose This study examined the anti-hepatocarcinogenic properties of QZACP, with a specific focus on its influence on the p21-activated secretory phenotype (PASP)-mediated immune surveillance , to elucidate the underlying molecular pathways involved in HCC. Materials and methods Cell proliferation was measured using the Cell Counting Kit-8, 5-ethynyl-2′-deoxyuridine, and clonogenic assays . The cell cycle was evaluated using flow cytometry, and senescence was identified by staining with senescence-associated beta-galactosidase (SA-β-gal). A primary liver cancer model produced by diethylnitrosamine was established in C57 BL/6 mice to assess the tumor-inhibitory effect of QZACP. The liver's pathological characteristics were examined using hematoxylin and eosin staining. PASP screening was performed using GeneCards, DisGeNet, Online Mendelian Inheritance in Man, and The Cancer Genome Atlas databases. Western blot analysis , enzyme-linked immunosorbent assay (ELISA), immunofluorescence staining, and Transwell migration assays were performed. Res...