Isolation, characterization, and pathogenicity of Fusarium species causing crown rot of wheat

作者：Guoping Ma, Heng Wang, Kai Qi, Liguo Ma, Bo Zhang, Yueli Zhang, Hang Jiang, Xuehong Wu, Junshan Qi · 发表于：Frontiers in Microbiology · 年份：2024 · DOI：10.3389/fmicb.2024.1405115 · 被引用次数：32 · 研究领域：Plant Pathogens and Fungal Diseases、Mycotoxins in Agriculture and Food、Plant-Microbe Interactions and Immunity

Fusarium crown rot (FCR) is one of the most important soilborne diseases affecting wheat production. To investigate the diversity of the pathogens causing this disease, 199 diseased wheat samples were collected from 13 cities in Shandong province. In total, 468 isolates were obtained, and from these isolates, 11 Fusarium species were identified based on phylogenetic analyses with the translation elongation factor-1α ( TEF-1α ), RNA polymerase II largest subunit ( RPB1 ), and RNA polymerase II second largest subunit ( RPB2 ) gene sequences. Of these Fusarium isolates, 283 were identified as Fusarium pseudograminearum and the remaining isolates were identified as Fusarium graminearum ( n = 113), Fusarium sinensis ( n = 28), Fusarium acuminatum ( n = 18), Fusarium incarnatum ( n = 13), Fusarium ipomoeae ( n = 5), Fusarium flocciferum ( n = 3), Fusarium proliferatum ( n = 2), Fusarium asiaticum ( n = 1), Fusarium culmorum ( n = 1), and Fusarium oxysporum ( n = 1), suggesting that F. pseudograminearum is the dominant pathogen of FCR of wheat in Shandong province. Pathogenicity tests demonstrated that all 11 Fusarium species could cause typical symptoms of FCR on wheat seedlings. The results of the study indicate that a greater diversity of Fusarium species can cause FCR of wheat in Shandong province than that has been previously reported. This is the first report in the world of Fusarium incarnatum , Fusarium ipomoeae , and Fusarium flocciferum as pathogens causing FCR in wheat.