Nitrogen-cycling microbial communities respond differently to nitrogen addition under two contrasting grassland soil types

作者：Baihui Ren, Xinwei Ma, Daiyan Li, Long Bai, Jiahuan Li, Jianxin Yu, Meng Meng, H. Li · 发表于：Frontiers in Microbiology · 年份：2024 · DOI：10.3389/fmicb.2024.1290248 · 被引用次数：29 · 研究领域：Soil Carbon and Nitrogen Dynamics、Microbial Community Ecology and Physiology、Biocrusts and Microbial Ecology

Introduction: The impact of nitrogen (N) deposition on the soil N-transforming process in grasslands necessitates further investigation into how N input influences the structural composition and diversity of soil N-cycling microbial communities across different grassland types. Methods: In this study, we selected two types of grassland soils in northwest Liaoning, temperate steppe and warm-temperate shrub, and conducted short-term N addition experiments using organic N, ammonium N, and nitrate N as sources with three concentration gradients to simulate N deposition. Illumina MiSeq sequencing technology was employed to sequence genes associated with N-cycling microbes including N-fixing, ammonia-oxidizing and denitrifying bacteria, and ammonia-oxidizing archaea. Results and discussion: The results revealed significant alterations in the structural composition and diversity of the N-cycling microbial community due to N addition, but the response of soil microorganisms varied inconsistent among different grassland types. Ammonium transformation rates had a greater impact on soils from temperate steppes while nitrification rates were more influential for soils from warm-temperate shrubs. Furthermore, the influence of the type of N source on soil N-cycling microorganisms outweighed that of its quantity applied. The ammonium type of nitrogen source is considered the most influential driving factor affecting changes in the structure of the microbial community involved in nitrogen tr...