DNA methylase 1 influences temperature responses and development in the invasive pest Tuta absoluta

作者：Yanhong Tang, Huifang Zhang, Huanqing Zhu, Siyan Bi, Xiaodi Wang, Shun‐Xia Ji, Jianhang Ji, Dongfang Ma, Cong Huang, Guifen Zhang, Nianwan Yang, Fanghao Wan, Zhichuang Lü, Wanxue Liu · 发表于：Insect Molecular Biology · 年份：2024 · DOI：10.1111/imb.12919 · 被引用次数：8 · 研究领域：Crustacean biology and ecology、Oil Palm Production and Sustainability、Insect symbiosis and bacterial influences

DNA methylase 1 (Dnmt1) is an important regulatory factor associated with biochemical signals required for insect development. It responds to changes in the environment and triggers phenotypic plasticity. Meanwhile, Tuta absoluta Meyrick (Lepidoptera: Gelechiidae)-a destructive invasive pest-can rapidly invade and adapt to different habitats; however, the role of Dnmt1 in this organism has not been elucidated. Accordingly, this study investigates the mechanism(s) underlying the rapid adaptation of Tuta absoluta to temperature stress. Potential regulatory genes were screened via RNAi (RNA interference), and the DNA methylase in Tuta absoluta was cloned by RACE (Rapid amplification of cDNA ends). TaDnmt1 was identified as a potential regulatory gene via bioinformatics; its expression was evaluated in response to temperature stress and during different development stages using real-time polymerase chain reaction. Results revealed that TaDnmt1 participates in hot/cold tolerance, temperature preference and larval development. The full-length cDNA sequence of TaDnmt1 is 3765 bp and encodes a 1254 kDa protein with typical Dnmt1 node-conserved structural features and six conserved DNA-binding active motifs. Moreover, TaDnmt1 expression is significantly altered by temperature stress treatments and within different development stages. Hence, TaDnmt1 likely contributes to temperature responses and organismal development. Furthermore, after treating with double-stranded RNA and exposing ...