Responses of nutrient utilization, rumen fermentation and microorganisms to different roughage of dairy buffaloes

作者：Shichun He, Ruiyun Zhang, Rongjiao Wang, Dongwang Wu, Sifan Dai, Zibei Wang, Tao Chen, Huaming Mao, Qing Li · 发表于：BMC Microbiology · 年份：2024 · DOI：10.1186/s12866-024-03342-0 · 被引用次数：13 · 研究领域：Ruminant Nutrition and Digestive Physiology、Genetic and phenotypic traits in livestock、Reproductive Physiology in Livestock

Abstract Dairy buffaloes are typically fed a high-forage, low-quality diet with high fiber. These conditions result in an inherent energy and protein inefficiency. In order to make full and rational use of feed resources and improve the production level and breeding efficiency of dairy buffaloes, the effects of various roughages on nutrient digestibility, ruminal fermentation parameters, and microorganisms in dairy buffaloes were studied in this experiment. Three ternary hybrid buffaloes, with an average body weight of 365 ± 22.1 kg, were selected and fitted with permanent rumen fistulas. They were fed six different diets, each consisting of 1 kg concentrate supplement and one of six types of roughage, including alfalfa hay (A diet), oat hay (O diet), whole corn silage (W diet), king grass (K diet), sugarcane shoot silage (S diet), and rice straw hay (R diet) according to an incomplete Latin square design of 3 × 6, respectively. The pre-feeding period of each period was 12 d. From day 13 to 15 was the official experimental period. During the prefeeding period, free feed intake for each roughage was determined, and during the experiment, the roughage was fed at 90% of the voluntary feed intake. Digestion and metabolism tests were carried out using the total manure collection method to determine the feed intake and fecal output of each buffalo, and to collect feed and fecal samples for chemical analysis. On day 15, rumen fluid samples were collected two hours after morning feed...