In Vivo Polymer Mechanochemistry with Polynucleotides

作者：Aman Ishaqat, Johannes Hahmann, Cheng Lin, Xiaofeng Zhang, Chuanjiang He, W Rath, Pardes Habib, Sabri E. M. Sahnoun, Khosrow Rahimi, Rostislav Vinokur, Felix M. Mottaghy, Robert Göstl, Matthias Bartneck, Andreas Herrmann · 发表于：Advanced Materials · 年份：2024 · DOI：10.1002/adma.202403752 · 被引用次数：21 · 研究领域：Force Microscopy Techniques and Applications、Lipid Membrane Structure and Behavior、Cellular Mechanics and Interactions

Polymer mechanochemistry utilizes mechanical force to activate latent functionalities in macromolecules and widely relies on ultrasonication techniques. Fundamental constraints of frequency and power intensity have prohibited the application of the polymer mechanochemistry principles in a biomedical context up to now, although medical ultrasound is a clinically established modality. Here, a universal polynucleotide framework is presented that allows the binding and release of therapeutic oligonucleotides, both DNA- and RNA-based, as cargo by biocompatible medical imaging ultrasound. It is shown that the high molar mass, colloidal assembly, and a distinct mechanochemical mechanism enable the force-induced release of cargo and subsequent activation of biological function in vitro and in vivo. Thereby, this work introduces a platform for the exploration of biological questions and therapeutics development steered by mechanical force.