Taxonomic identification and antagonistic activity of Streptomyces luomodiensis sp. nov. against phytopathogenic fungi

作者：Dengfeng Qi, Qiao Liu, Liangping Zou, Miaoyi Zhang, Kai Li, Yankun Zhao, Yufeng Chen, Junting Feng, Dengbo Zhou, Yongzan Wei, Wei Wang, Lu Zhang, Jianghui Xie · 发表于：Frontiers in Microbiology · 年份：2024 · DOI：10.3389/fmicb.2024.1402653 · 被引用次数：8 · 研究领域：Plant Disease Resistance and Genetics、Plant Pathogens and Fungal Diseases、Plant-Microbe Interactions and Immunity

Banana wilt caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is a devastating fungal disease. Biocontrol strategies hold immense potential for inhibiting the spread of Foc TR4. Here, 30 actinobacteria were isolated from soils and screened for their antagonistic activity against Foc TR4. Strain SCA4-21 T was selected due to its strongest antagonistic activity against Foc TR4. Strain SCA4-21 T also exhibited strong antagonistic activity against the other eight phytopathogenic fungi. The strain was identified as the genus Streptomyces according to its physiological, biochemical, and phenotypic characteristics. The phylogenetic trees of 16S rRNA sequences demonstrated that strain SCA4-21 T formed a subclade with S. iranensis HM 35 T and/or S. rapamycinicus NRRL B-5491 T with low bootstrap values. Considering that 16S rRNAs did not provide sufficient resolution for species-level identification, the whole genome of strain SCA4-21 T was sequenced. Multilocus sequence analysis (MLSA) based on five housekeeping gene alleles ( atpD , gyrB , recA , rpoB , and trpB ) revealed that strain SCA4-21 T clustered into S. hygroscopicus subsp. hygroscopicus NBRC 13472 T with 100% of bootstrap value. The analysis of the genome-based phylogeny also approved the results. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) were 91.26 and 44.30%, respectively, with values below the respective species level threshold of 95 and 70%. Hence, strain SCA 4–21 T r...