P-hydroxybenzaldehyde protects Caenorhabditis elegans from oxidative stress and β-amyloid toxicity

作者：Xingzhi Yu, Jie Tao, Tian Xiao, Xiaohua Duan · 发表于：Frontiers in Aging Neuroscience · 年份：2024 · DOI：10.3389/fnagi.2024.1414956 · 被引用次数：13 · 研究领域：Biological and pharmacological studies of plants、Circadian rhythm and melatonin、Medicinal Plants and Neuroprotection

Introduction Gastrodia elata is the dried tuber of the orchid Gastrodia elata Bl. It is considered a food consisting of a source of precious medicinal herbs, whose chemical composition is relatively rich. Gastrodia elata and its extracted fractions have been shown to have neuroprotective effects. P -hydroxybenzaldehyde ( p -HBA), as one of the main active components of Gastrodia elata , has anti-inflammatory, antioxidative stress, and cerebral protective effects, which has potential for the treatment of Alzheimer’s disease (AD). The aim of this study was to verify the role of p -HBA in AD treatment and to investigate its mechanism of action in depth based using the Caenorhabditis elegans ( C. elegans ) model. Methods In this study, we used paralysis, lifespan, behavioral and antistress experiments to investigate the effects of p -HBA on AD and aging. Furthermore, we performed reactive oxygen species (ROS) assay, thioflavin S staining, RNA-seq analysis, qPCR validation, PCR Array, and GFP reporter gene worm experiment to determine the anti-AD effects of p -HBA, as well as in-depth studies on its mechanisms. Results p -HBA was able to delay paralysis, improve mobility and resistance to stress, and delay aging in the AD nematode model. Further mechanistic studies showed that ROS and lipofuscin levels, Aβ aggregation, and toxicity were reduced after p -HBA treatment, suggesting that p -HBA ameliorated Aβ-induced toxicity by enhancing antioxidant and anti-aging activity and inhibi...