Simultaneous detection of 5-methylcytosine and 5-hydroxymethylcytosine at specific genomic loci by engineered deaminase-assisted sequencing

作者：Neng‐Bin Xie, Min Wang, Tong‐Tong Ji, Xia Guo, Fang‐Yin Gang, Ying Hao, Li Zeng, Yafen Wang, Yu‐Qi Feng, Bi‐Feng Yuan · 发表于：Chemical Science · 年份：2024 · DOI：10.1039/d4sc00930d · 被引用次数：20 · 研究领域：Epigenetics and DNA Methylation、RNA modifications and cancer、Cancer-related molecular mechanisms research

values, we can deduce the precise levels of C, 5mC, and 5hmC at particular genomic locations. We successfully used EDA-seq to simultaneously measure C, 5mC, and 5hmC at specific loci within human lung cancer tissue and their normal counterpart. The results from EDA-seq demonstrated a strong concordance with those obtained from the combined application of BS-seq and ACE-seq methods. EDA-seq eliminates the need for bisulfite treatment, DNA oxidation or glycosylation and uniquely enables simultaneous quantification of C, 5mC and 5hmC at the same genomic locations.