Strategic approach for HPLC Method Development and Validation: Review

作者：Amitkumar J. Vyas, Dhruvi U. Parmar, Ashok B. Patel, Ajay I. Patel, Ashvin Dudhrejiya, Sunny Shah · 发表于：Asian Journal of Research in Pharmaceutical Sciences · 年份：2024 · DOI：10.52711/2231-5659.2024.00011 · 被引用次数：6 · 研究领域：Analytical Methods in Pharmaceuticals、Analytical Chemistry and Chromatography、Analytical chemistry methods development

High performance liquid chromatography (HPLC) is an analytical technique which is adept to separate, detect and quantify numerous pharmaceuticals and their related degradants. It is also used to identify and measure synthetic drugs, to minimise other impurities at the time of separation, and to separate manufactured medicines from drug-related contaminants. To improve the procedure, a number of chromatographic parameters were assessed. It is necessary to identify the best mobile phase, column, detector, buffer, wavelength, and gradient for the drug's compatibility and stability with degradants and contaminants. This study discusses the significance of RP-HPLC in the development of analytical methods, their techniques, and a brief overview of the key chromatographic parameters that must be tuned for effective method development.