Magnetic field-assisted surface engineering technology for active regulation of Fe3O4 medium to enable the laccase electrochemical biosensing of catechol with visible stripe patterns

作者：Feng Wang, Jie Zhang, Jie Zhang, Ling Xu, Anzhou Ma, Guoqiang Zhuang, Shuhao Huo, Bin Zou, Jingya Qian, Yi Cui, Wen Zhang · 发表于：Analytica Chimica Acta · 年份：2024 · DOI：10.1016/j.aca.2024.342739 · 被引用次数：8 · 研究领域：Electrochemical sensors and biosensors、Advanced biosensing and bioanalysis techniques、Enzyme-mediated dye degradation

BACKGROUND: Catechol (CC), a prevalent phenolic compound, is a byproduct in various agricultural, chemical, and industrial processes. CC detection is crucial for safeguarding water quality and plays a pivotal role in enhancing the overall quality of life of individuals. Electrochemical biosensors exhibit rapid responses, have small sizes, and can be used for real-time monitoring. Therefore, the development of a fast and sensitive electrochemical biosensor for CC detection is crucial. RESULT: nanoparticles as medium and optimized by applying a magnetic field. This research proposes a unique strategy for biosensor enhancement by actively controlling the distribution of magnetic materials on the electrode surface through the application of a magnetic field, resulting in a visibly alternating stripe pattern. This approach effectively disperses magnetic particles, preventing their aggregation and reducing the boundary layer thickness, enhancing the electrochemical response of the biosensor. After fabrication condition optimization, CC is successfully detected using this biosensor. The fabricated sensor exhibits excellent performance with a wide linear detection range of 10-1000 μM, a low detection limit of 1.25 μM, and a sensitivity of 7.9 μA/mM. The fabricated sensor exhibits good selectivity and reliable detection in real water samples. In addition, the laccase-based sensor has the potential for the fast and accurate monitoring of CC in olive oil. SIGNIFICANCE: The magnetic fiel...