Exploring the gut microbiota-hippocampus-metabolites axis dysregulation in sepsis mice

作者：Fangqiang Song, Qinglun Li, J. Cui, Jianhua Wang, Shuai Xiao, Bo Yu, Yanqi Sun, Wenke Song, Linlin Wu, Zhou Yongqin · 发表于：Frontiers in Microbiology · 年份：2024 · DOI：10.3389/fmicb.2024.1302907 · 被引用次数：5 · 研究领域：Gut microbiota and health、Tryptophan and brain disorders、Intensive Care Unit Cognitive Disorders

Background Sepsis is commonly associated with a sudden impairment of brain function, thus leading to significant rates of illness and mortality. The objective of this research was to integrate microbiome and metabolome to reveal the mechanism of microbiota-hippocampus-metabolites axis dysfunction in a mouse model of sepsis. Methods A mouse model of sepsis was established via cecal ligation and puncture. The potential associations between the composition of the gut microbiota and metabolites in the hippocampus of mice with sepsis were investigated by combining 16S ribosomal RNA gene sequencing and ultra-high-performance liquid chromatography tandem mass spectrometry. Results A total of 140 differential metabolites were identified in the hippocampal tissues of mice with sepsis when compared to those of control mice. These differential metabolites in mice with sepsis were not only associated with autophagy and serotonergic synapse, but also involved in the metabolism and synthesis of numerous amino acids. At the phylum level, the abundance of Bacteroidota was increased, while that of Firmicutes ( Bacillota ) was decreased in mice with sepsis. At the genus level, the abundance of Alistipes was increased, while that of Lachnospiraceae_NK4A136_group was decreased in mice with sepsis. The Firmicutes ( Bacillota )/ Bacteroidota (F/B) ratio was decreased in mice with sepsis when compared to that of control mice. Furthermore, the F/B ratio was positively correlated with 5′-methylthioad...