The Dark Side of the pollen: BSA-seq identified genomic regions linked to male sterility in globe artichoke

作者：Matteo Martina, Aldana Zayas, Ezio Portis, Giovanna Di Nardo, Maria Francesca Polli, Cinzia Comino, Gianfranco Gilardi, Eugenia Martín, Alberto Acquadro · 发表于：BMC Plant Biology · 年份：2024 · DOI：10.1186/s12870-024-05119-z · 被引用次数：4 · 研究领域：Cynara cardunculus studies、Botanical Studies and Applications

Abstract Globe artichoke ( Cynara cardunculus var. scolymus ; 2n = 2x = 34) is a food crop consumed for its immature flower heads. Traditionally, globe artichoke varietal types are vegetatively propagated. However, seed propagation makes it possible to treat the crop as annual, increasing field uniformity and reducing farmers costs, as well as pathogens diffusion. Despite globe artichoke’s significant agricultural value and the critical role of heterosis in the development of superior varieties, the production of hybrids remains challenging without a reliable system for large-scale industrial seed production. Male sterility (MS) presents a promising avenue for overcoming these challenges by simplifying the hybridization process and enabling cost-effective seed production. However, within the Cynara genus, genic male sterility has been linked to three recessive loci in globe artichoke, with no definitive genetic mechanism elucidated to date. A 250 offsprings F 2 population, derived from a cross between a MS globe artichoke and a male fertile (MF) cultivated cardoon ( C. cardunculus var. altilis ) and fitting a monogenic segregation model (3:1), was analyzed through BSA-seq, aiming at the identification of genomic regions/genes affecting male sterility. Four QTL regions were identified on chromosomes 4, 12, and 14. By analyzing the sequence around the highest pick on chromosome 14, a cytochrome P450 ( CYP703A2 ) was identified, carrying a deleterious substitution (R/Q) fixed in...